Monday, December 28, 2009

Enable X11 Forwarding in MacOS

e.g. to use the GIMP,
edit field `X11Forwarding' in file `/etc/sshd_config'

See: http://developer.apple.com/qa/qa2004/qa1383.html.

Showing all files in MacOS Finder


defaults write com.apple.finder ShowAllFiles TRUE
killall Finder

Monday, December 21, 2009

Normalizing Affymetrix Data with bioconductor


#!/usr/bin/env Rscript
# Normalize a set of CEL files

## Note: To use biocLite packages (i.e. 'affy,' 'rma()', ...), it may be
# necessary to do a one-time [re-]install of the related packages. This can be
# done with the following sequence:

# > source("http://bioconductor.org/biocLite.R"); # Tell R where to download package info from
# > biocLite(); # Update biocLite package grounds. This turns out to be necessary 1-time step before a call to rma(...), which requires up-to-date biocLite library functions
# > biocLite("affy"); # d/l, install 'affy' package (1-time req. only)

library("affy");
# We'll be using this library

# Read CEL files
filenames <- list.files("path/to/CEL", full.names=TRUE);
affy.data <- ReadAffy(filenames=filenames, verbose=TRUE);

## Normalize using RMA
write.table( exprs( rma(affy.data, verbose=TRUE)), file="rma.matrix", sep='\t', quote=FALSE); # file="" to write to stdout, but watch for verbose output from rma(...) and/or ReadAffy(...)

## Using Quantile Norm (this post is edited--the old way didn't collapse measurements per probe)
library("affyPLM")
eset <- threestep (affy.data, background.method="IdealMM", normalize.method="quantile", summary.method="tukey.biweight" )
write.table(eset, file="qnorm.matrix", sep='\t', quote=FALSE)
# for some reason, this transposes the data and adds an "X" in front of every numeric probe ID?

Friday, December 4, 2009

Recursively downloading from an FTP site


  1. ncftp

    $ sudo port install ncftp
    $ cd ~/Downloads/uci
    $ time ncftpget -R -v -u "anonymous" ftp.ics.uci.edu ~/Downloads/uci pub/machine-learning-databases


    So far, I'm not finding this to be doing a very good job of being recursive.

    Source:
    http://www.cyberciti.biz/tips/linux-download-all-file-from-ftp-server-recursively.html

  2. wget


    $ sudo port install wget
    $ cd ~/Downloads/uci2
    $ time wget -r ftp://ftp.ics.uci.edu/pub/machine-learning-databases

    Source: http://www.editcorp.com/Personal/Lars_Appel/wget/v1/wget_7.html, http://www.gnu.org/software/wget/manual/html_node/Recursive-Download.html.
    See also: gwget


Tuesday, December 1, 2009

Installing LaTeX on a Mac

Download and unzip MacTeX.mpkg.zip from http://www.tug.org/mactex
and run the mkpg.

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